Genome Editing Techniques & Applications

Course Information

Course Code

GETA110

Instruction Method

Online material supports the application of knowledge in 2 week hands-on laboratory opportunity.

 

Fee

See below for registration information.

Length

2 weeks

Instructor(s)

Jason De Melo, Ph.D.

Learner Outcomes

Genome editing with CRISPR/Cas is revolutionizing biotechnology. In this course, learners will discover many of the applications for CRISPR/Cas and gain hands-on experience editing DNA.

  • Understanding of aseptic technique with regards to bacterial handling
  • Understand the theoretical principles involved in molecular cloning and genome editing
  • Successfully map and execute a simple cloning protocol for insertion
  • Create cloning protocols
  • Successfully design and then assess the success or failure of experiments
  • Identify sources of error
  • Identify appropriate experimental controls
  • Acquire practical knowledge of how to edit the genome from simple bacteria to larger organism

Course Objectives

Upon completion of the course, the learner will be able to:

  1. Understand the theoretical principles involved in molecular cloning and genome editing
  2. Design and execute a simple cloning protocol for the insertion of a short oligonucleotide into a larger plasmid fragment
  3. Edit the genome of a simple bacteria and scale up this to work with larger organisms
  4. Identify sources of error using appropriate experimental controls

Evaluation Method

  • Attendance mandatory for all days
  • Lab techniques and experimental outcomes (60%)
  • Experimental Design Project (40%) Design model system and presentation

Throughout the wet-lab portion of the course, learners will be asked to demonstrate a proficiency in a number of standard laboratory techniques. These include but not limited to:

  • Reagent preparation
  • Aseptic technique
  • Pipetting
  • Concentration calculations
  • DNA sequence analysis
  • Cloning of plasmid
  • Generation of antibiotic resistant bacteria

 

Intended For

Current and future Research Technicians, Graduate Students, Post-doctoral Fellows and Undergraduate students who are/will be working with CRISPR/Cas and genome editing.

Text Book

Materials provided

Location

Michener Institute of Education at UHN, ST. Patrick Street Campus

Prerequisites

  • Introductory microbiology laboratory course from a post-secondary institution or adequate training in aseptic technique
  • Completion of the Michener Institute of Education at UHN’s Biosafety and WHMIS training prior to start of the course (will be made available after registration)

Topics Covered

In class:

  • Background of CRISPR/Cas9
  • Designing a CRISPR/Cas9 experiment
  • Applications of CRISPR/Cas9: Genetic Screens, Therapeutics, Synthetic Biology
  • Ethics of Genome Editing

In laboratory:

  • Cloning of a sgRNA plasmid
  • Genome editing in bacteria
  • Validation of genetic modifications: T7 Endonuclease assay, Sanger sequencing and TIDE analysis

GETA110 – Genome Editing Techniques & Applications

Courses

CodeCourse TitleFormatTuitionDate(s)Register ByAvailability
GETA110-SLAIn Class$999.00Aug-13-2018 to Aug-24-2018Aug-10-2018Open