Genome Editing Techniques & Applications

Course Information

Course Code

GETA110

Instruction Method

Hybrid Learning – Online material supports the application of knowledge in weekly hands-on laboratory opportunity.

July 29, 2019 – access to online material

Must be available to attend 5 on site days 9-4pm

August 8, 2019– on site lab 1 at Michener St. Patrick Campus Room 1441

August 15, 2019 on site lab 2a at Michener St. Patrick Campus Room 1441

August 21, 2019 on site lab 2b at Michener St. Patrick Campus Room 1441

August 22, 2019 on site lab 3 at Michener St. Patrick Campus Room 1441

August 29, 2019 on site in-class at Michener St. Patrick Campus Room 1441

 

Fee

See below for registration information.

Length

5 weeks

Instructor(s)

Jason De Melo, Ph.D.

Learner Outcomes

Genome editing using CRISPR technology represents one of the most innovative areas of biotechnology. Over the last 10 years, the potential to produce transformative breakthroughs in both human health and agriculture has grown drastically and the advent of genome editing and CRISPR is at its forefront. In this course learners will learn about this emerging technology, gain practical experience editing model organisms and discuss the many applications and implications to medicine, agriculture and biotechnology.

 

Course Objectives

At the end of this course, learners will:

  • Develop a theoretical understanding of genome editing using CRISPR/Cas9
  • Design experimental protocols to edit simple and complex organisms
  • Gain practical, hands-on-wet-lab experience editing model organisms
  • Create an experimental plan to address a research question using CRISPR

 

Evaluation Method

  • Attendance mandatory for lab days- August 8, 15, 21, 22, and 29
  • Lab techniques and experimental outcomes (60%)
  • Experimental Design Project (40%) Design model system and presentation

Throughout the wet-lab portion of the course, learners will be asked to demonstrate a proficiency in a number of standard laboratory techniques. These include but not limited to:

  • Reagent preparation
  • Aseptic technique
  • Pipetting
  • Concentration calculations
  • DNA sequence analysis
  • Cloning of plasmid
  • Generation of antibiotic resistant bacteria

 

Intended For

Current and future Research Technicians, Graduate Students, Post-doctoral Fellows and Undergraduate students who are/will be working with CRISPR/Cas and genome editing.


Note:

GETA110- This offering will be a combination of online learning with weekly on site laboratory sessions.

Weekly online assignments will be validated by the on site laboratory work.  This is truly hybrid learning. There must be commitment to completing and bringing the online work to the scheduled laboratory days. The scheduled days are spread throughout the 5 weeks so that it is easier to incorporate into work schedules.

Register early as there are limited spots.

Text Book

Materials provided

Location

Michener Institute of Education at UHN, ST. Patrick Street Campus

Prerequisites

  • Introductory microbiology laboratory course from a post-secondary institution or adequate training in aseptic technique
  • Completion of the Michener Institute of Education at UHN’s Biosafety and WHMIS training prior to start of the course (will be made available after registration)

Topics Covered

In class:

  • Background of CRISPR/Cas9
  • Designing a CRISPR/Cas9 experiment
  • Applications of CRISPR/Cas9: Genetic Screens, Therapeutics, Synthetic Biology
  • Ethics of Genome Editing

In laboratory:

  • Cloning of a sgRNA plasmid
  • Genome editing in bacteria
  • Validation of genetic modifications: T7 Endonuclease assay, Sanger sequencing and TIDE analysis

GETA110 – Genome Editing Techniques & Applications

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